蛋白质纯化方法经典攻略！
<div style="max-width:100%;overflow:hidden;"><section data-mpa-powered-by="yiban.io"><section><section><section><section><section><section data-autoskip="1"><section data-support="96编辑器" data-style-id="35021"><section><p style="text-align: center;margin-bottom: 0em;"><br></p><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;text-align: center;visibility: visible;"></section><section style="margin: 0px;padding: 14px;outline: 0px;max-width: 100%;box-sizing: border-box;overflow-wrap: break-word !important;border-bottom: 2px solid rgb(255, 183, 110);background-color: rgb(255, 242, 235);visibility: visible;"><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;color: rgb(93, 34, 18);letter-spacing: 2px;font-size: 14px;line-height: 1.75em;transform: rotateZ(0deg);visibility: visible;"><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;line-height: 1.5em;visibility: visible;"><span style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;font-size: 14px;letter-spacing: 1px;visibility: visible;">在结构研究和体外生物化学分析等很多实验应用中都需要用到纯化蛋白质。蛋白质可以从组织中获取，亦或更经常的是从模式生物中过量表达获得，如细菌、酵母或哺乳动物细胞培养等。蛋白质纯化主要是根据它们各不相同的物理性质来从原料进行分离，其目的就是希望能浪费最少的杂蛋白，获得最多的功能蛋白质。一个好的蛋白质的纯化过程必须要将其纯化工艺步骤优化至最少。</span></section></section></section></section></section><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;line-height: 1.5em;visibility: visible;"><br style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible;"></section></section></section></section></section><section data-support="96编辑器" data-style-id="35720" style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible;"><section style="margin: 10px 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;text-align: center;line-height: 1.5em;visibility: visible;"><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;display: inline-block;visibility: visible;"><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible;"><section style="margin: 0px;padding: 6px;outline: 0px;max-width: 100%;box-sizing: border-box;overflow-wrap: break-word !important;background-image: vw/640?wx_fmt=png;background-position: 0% center;background-repeat: no-repeat;background-size: 100% 100%;visibility: visible;"><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box;overflow-wrap: break-word !important;border-width: 1px;border-style: solid;border-color: rgb(151, 173, 119);background-color: rgb(254, 254, 254);visibility: visible;"><section style="margin: 0px;padding: 3px 15px;outline: 0px;max-width: 100%;box-sizing: border-box;overflow-wrap: break-word !important;font-size: 18px;color: rgb(57, 76, 14);letter-spacing: 2px;visibility: visible;"><p style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;clear: both;min-height: 1em;visibility: visible;"><strong style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible;"><span style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;letter-spacing: 1px;font-size: 16px;visibility: visible;">蛋白质纯化工艺的建立</span></strong></p></section></section></section></section></section></section></section><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible;"><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible;"><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;line-height: 1.5em;visibility: visible;"><span style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;color: rgb(255, 41, 65);visibility: visible;"><strong style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible;"><span style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;font-size: 14px;letter-spacing: 1px;visibility: visible;">建立蛋白质纯化工艺时，最重要的是需要考虑纯化得到的蛋白质应能满足其后续应用要求。</span></strong></span><span style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;font-size: 14px;letter-spacing: 1px;visibility: visible;">蛋白质的数量及纯度都必须满足实验分析要求。而且，因为后续研究需要的是保持良好折叠结构的活性蛋白质，因此蛋白质行为的相关信息也需要考虑。在纯化及后续的保存过程中，很多处理方法都会对蛋白质的性质产生影响，如蛋白质的去折叠、聚集、降解和失活等。制定详细计划，在最短时间内完成蛋白质纯化，并在最稳定的条件下进行保存才算是成功地完成了其整个纯化过程。<br style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible;"></span></section><section style="margin: 0px;padding: 0px;outline: 0px;max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;line-height: 1.5em;visibility: visible;"><br 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